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[Fluorescence and HTS]
[Cell Assays, Pharmacol.] [Biosensors] [Immunochem., Liposomes] [Membrane Biophysics] [Chemical Physics of Water] |
These publications involve microphysiometry, a biosensor-based technology that detects cellular responses to pharmacological and toxic agents by monitoring changes in the rate of cellular metabolism in real time. Owicki, J.C., Parce, J.W., Kercso, K.M., Sigal,
G.B., Muir, V.C., Venter, J.C., Fraser, C.M., & McConnell, H.M. (1990) Continuous Monitoring of Receptor-Mediated
Changes in the Metabolic Rates of Living Cells. Proc. Natl. Acad. Sci. U.S.A. 87:4007-4011. Wada, H.G., Owicki, J.C., and Parce, J.W. (1991) Cells on Silicon: Bioassays with a
Microphysiometer. Clinical Chemistry 37: 600-601. Bruner, L.H., Kercso, K.M., Owicki, J.C., Parce,
J.W., & Muir, V.C. (1990) Testing
Ocular Irritancy In Vitro with the Silicon Microphysiometer. Toxicology In
Vitro 5:277-284. Parce, J.W., Owicki, J.C., Wada, H.G., & Kercso,
K.M. (1991) Cells on Silicon: The
Microphysiometer. pp. 97-106 In:
Alternative Methods in Toxicology, Volume 8:
Mary Ann Liebert, Inc., New York, NY. Owicki, J.C., & Parce, J.W. (1992) Biosensors Based on the Energy Metabolism of
Living Cells: The Physical Chemistry and Cell Biology of Extracellular
Acidification. Biosensors and
Bioelectronics, 7:255-272. Raley-Susman, K.M., Miller, K.M., Owicki, J.C.,
& Sapolsky, R.M. (1992) Effects of
Excitotoxin Exposure on Metabolic Rate of Primary Hippocampal Cultures: Application of Silicon Microphysiometry to
Neurobiology. J. Neurosci.
12:773-780. Wada, H.G., Owicki, J.C., Bruner, L.H., Miller,
K.R., Raley-Susman, K.M., Panfili, P.R., Humphries, G.M.K., & Parce, J.W. (1992)
Measurement of Cellular Responses to
Toxic Agents using a Silicon Microphysiometer.
AATEX 1:154-164. Baxter, G.T., Miller, D.L., Kuo, R.C., Wada, H.G.,
& Owicki, J.C. (1992) PKCe is
involved in GM-CSF Signal Transduction: Evidence from Microphysiometry and
Antisense Oligonucleotide Experiments. Biochemistry
31:10950-10954. Miller, D.L., Olson, J.C., Parce, J.W., &
Owicki, J.C. (1993) Cholinergic
Stimulation of the Na+/K+
ATPase as Revealed by Microphysiometry.
Biophys. J. 64:813-823 Kuo, R.C., Baxter, G.T., Alajoki, L., Miller, D.L.,
Libby, J.M., & Owicki, J.C. (1993) A
Metabolic View of Receptor Activation in Cultured Cells Following
Cryopreservation. Cryobiology
30:386-395. Harvell, J.D., Owicki, J.C., & Maibach, H.I.
(1994) The Silicon Microphysiometer: Use in Ocular and Cutaneous Toxicity
Testing. Alt. Meth. Toxicol.
10:79-82. Baxter, G.T., Young, M.-L., Miller, D.L., &
Owicki, J.C. (1994) Using
Microphysiometry to Study the Pharmacology of Exogenously Expressed m1 and m3
Muscarinic Receptors. Life Sciences
55:573-583. Thibodeau, A., Kuo, R.C., Crothers, J.M., Jr., Yao,
X., Owicki, J.C., & Forte, J.G. (1994) Direct
Measurement of Extracellular Proton Flux from Isolated Gastric Glands. Am.
J. Physiol. 267: C1473-C1482. Salon, J.A., & Owicki, J.C. (1995) Real-Time Measurements of Receptor
Activity: Applications of
Microphysiometric Techniques to Receptor Biology. In: Methods in Neuroscience:
Receptor Molecular Biology (Ed. Sealfon, Academic, NY), vol. 25, pp.
201-224, invited chapter.
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